Home > Products > ELISA test > medical diagnostic reagent HIV 1/2 aids Elisa Test kit
medical diagnostic reagent HIV 1/2 aids Elisa Test kit
- 10000 Box / Boxes per Month
- tianjin or beijing
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Product Details
Type: | Blood Testing Equipments | Brand Name: | Bioneovan | Place of Origin: | Beijing, China |
Model Number: | in vitro diagnostic |
Product Description
Specifications
1.EIA kit is the third generation double antigen sandwich method for the detection of circulating antibodies2.immunoassay HIV
Item | Description | 96T | 480T |
1 | Micro titer Well | 1 | 5 |
2 | Sample Diluents | 6ml | 30ml |
3 | Enzyme Conjugate | 12ml | 60ml |
4 | Negative Control | 1ml | 5ml |
5 | Positive Control(HIV-1) | 1ml | 5ml |
6 | Positive Control(HIV-2) | 1ml | 5ml |
7 | Wash Buffer Concentrate (20x) | 30ml | 150ml |
8 | Substrate Solution A | 6ml | 30ml |
9 | Substrate Solution B | 6ml | 30ml |
10 | Stop Solution | 6ml | 30ml |
11 | User manual | 1 copy | 5 copy |
HBeAg ELISA Kit
Catalog No.:BE103A
Enzyme-linked immunosorbent assay for the detection HBeAg in serum or plasma
INTENDED USE |
HBeAg EIA is a qualitative enzyme immunoassay for the detection of HBeAg in human serum or plasma.
SUMMARY AND PRINCIPLE OF THE TEST |
The Hepatitis B e antigen is found to be related to Hepatitis B viral infection. HBeAg is usually found in the early phase of Hepatitis B viral infection. The titer of HBeAg rises rapidly during the period of virus replication. The presence of HBeAg correlates with increasing numbers of infectious viruses (Dane particles) and the presence of viral specific DNA polymerase in serum. During the HBeAg positive stage, hepatitis B patients are at increasing risk of transmitting the virus to their contacts. Persistent presence of HBeAg in the hepatitis B virus carrier is often associated with chronic active hepatitis.
The HBeAg EIA is a sandwich immunoassay, which employs monoclonal and polyclonal antibodies specific for HBeAg. Microtiter wells are coated with monoclonal antibodies specific for HBeAg. A serum specimen is added to the antibody coated microtiter wells together with enzyme conjugated polyclonal antibodies. HBeAg, if present, will form an antibody-HBeAg-antibody-enzyme complex. The plate is then washed to remove unbound materials. Finally, a solution of substrate is added to the wells and incubated. A blue color will develop in proportion to the amount of HBeAg present in the specimen. The enzyme substrate reaction can be stopped and the result is visualized by the naked eye or obtained by EIA plate reader for absorbance at wavelength of 450 nm (450 nm/630 nm).
REAGENTS |
Materials provided with the kits:
- 1. Microtiter well coated with monoclonal anti-HBe: 96 tests in one bag.
- 2. Negative Control: 0.5ml HBeAg Negative Control.
- 3. Positive Control: 0.5ml HBeAg Positive Control.
- 4. Enzyme Conjugate:.6 ml containing HRP-conjugated-anti-HBe
- 5. Wash Buffer Concentrate (20 x): 20ml for 96 tests. The buffer should be diluted 20 times with distilled water before use.
- 6. Substrate Solution A: 6 ml HRP Substrate for 96 tests.
- 7. Substrate Solution B: 6 ml TMB Chromagen Substrate for 96 tests.
- 8. Stop Solution: One bottle of 6ml 2N Sulfuric Acid
Materials required but not provided:
- 1. Precision pipettes: 0.02, 0.05, 0.10, 0.15, 0.20, and 1.0 ml.
- 2. Disposable pipette tips.
- 3. Distilled water.
- 4. HumidifiedBox capable of maintaining 37°C
- 5. Absorbent paper or paper towel.
- 6. Micro titer plate or strip-well washer
- 7. Micro titer plate reader.
PRECAUTION FOR USERS |
- 1. For in-vitro diagnostic use only.
- 2. Do not use kit beyond expiration date.
- 3. Do not mix components from kits with different lot number.
- 4. Avoid microbial contamination of reagents.
- 5. Do not pipet reagent by mouth and no smoking or eating while performing assays.
- 6. Wear gloves during the whole process and avoid reagents or specimen spilling-out.
- 7. Wipe up the spills using 5% hypochlorite solution.
- 8. Decontaminate all liquids or solid wastes before deposing.
SPECIMEN COLLECTION AND PREPARATION |
Serum should be prepared from a whole blood specimen obtained by acceptable medical techniques. Either serum or plasma can be used in this test. Remove serum or plasma from the clot or blood cells as soon as possible to avoid hemolysis. Specimen with extensive particulate should be clarified by centrifugation prior to use. Specimen frozen at -20°C or colder may be used. Avoid repeated freeze thaw.
STORAGE OF TEST KIT |
Unopened test kits should be stored at 2-8C. DO NOT FREEZE KIT COMPONNETS. The microtiter plate should be kept in a sealed bag to minimize exposure to damp air. Use up the reagents as soon as possible after the kit is unpacked.
Contact Us
- Bioneovan Co., Ltd.
- Contact nameSteven Tan
- Phone86-010-69255832
- AddressNo. 18, Keyuan Road, Industry Development Zone
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