One step Melamine Test kit(Melamine Test/lateral flow immunoassay/ISO9001/ISO1345 certified)

Specifications

Quicking Melamine Test is qualitative detection of Melamine in 5 - 10 min

Introduction

Specifications

Principle: Competitive lateral flow immunochromatographic assay

Intended use: Detection of Melamine

Specimen: Milk, milk powder, yogurt, feed and egg      

Reading time: 5 - 10 min

Shelf life: 18 months

Storage: 2- 30 °C. No freezing.

Packing size: 10 tests / box

Specimen	Cut-off
Milk	1.0 ppm
Milk Powder	2.5 ppm
Yogurt	1.0 ppm
Feed	2.5 ppm
Egg	1.0 ppm

 

Advantages

l Quicking Melamine Test enables detect Melamine in specimen in low detection limit.

l Quicking Melamine Test is a quick and easy testing tool that can be performed in just 10 minutes.

l Quicking Melamine Test can be stored for 18 months at room temperature, between 2 and 30°C.

 

Instructions

Kit component

-          10×foil pouches each containing one cassette and a desiccant

-          1×assay buffer (50 mL)

-          10×centrifugal tubes (1.5 mL)

-          Products Manual

 

Sample extraction

1.1   Milk (1.0 ppm)

Transfer 0.2 mL of milk sample into a centrifugal tube. (Tip: The volume of milk sample reaches the scale of the small pipette provided in the foil pouch). Add 0.6 mL of assay buffer into the tube and mix well with the milk sample. Use the mixture in the assay.  

1.2   Milk powder (2.5 ppm)

Dissolve 0.5 g of milk powder with 5 mL of 60 °C tap water. Keep it cool to room temperature. Use the liquid milk for running the assay.

1.3   Yogurt (1.0 ppm)

Transfer 0.2 mL of yogurt sample into a centrifugal tube. (Tip: The volume of yogurt sample reaches the scale of the small pipette provided in the foil pouch). Add 0.6 mL of assay buffer into the tube and mix well. Do centrifugation at 4000 rpm for 1 min. Take the supernatant for assay.

1.4    Feed (2.5 ppm)

Grind a representative sample to the particle size of fine instant coffee (50% passes through a 20 mesh screen). Weigh out 0.5 g ground portion of the sample into a sample tube. Add 4 mL of assay buffer into the tube and shake for 5 min. Use the supernatant in the assay. (If it is cloudy, do a centrifugation at 4000 rpm for 1 min.)

1.5   Egg (2.5 ppm)

Suck 0.2 mL of egg sample into a centrifugal tube. Add 0.8 mL of assay buffer into the tube and mix well with the sample. Use the mixture for assay.

 

Test procedure

-          Take out the cassette from the foil pouch and place it horizontally.

-          Gradually drip 3 drops of sample extraction into the assay sample hole.

-          Interpret the result in 5 - 10 min. Result after 10 min is considered as invalid.

 

Interpretation of results

Positive: Only one clear band in C zone indicates a positive result. Positive shows that the concentration of Melamine is at or above the cut-off value in the sample.
Negative: The presence of both clear band in C zone and T zone.

Invalid: No colored band appears in C zone.

 

 

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