Bovine Rapid Test BTB Ag

Bovine Tuberculosis Ab Test
Cat No.: W81096

1. INTENDED USE

Quicking Bovine Tuberculosis Ab Rapid Test is a sandwich lateral flow immunochromatographic assay for the qualitative detection of Bovine Tuberculosis antibody (BTB Ab) in serum or plasma.

Assay Time: 5 min

Sample: Serum or plasma

2. PRINCIPLE OF THE ASSAY

Quicking Bovine Tuberculosis Ab Rapid Test is based on sandwich lateral flow immunochromatographic assay. The test device has a testing window. The testing window has an invisible T (test) zone and C (control) zone. When sample is applied into the sample hole on the device, the liquid will laterally flow on the surface of the test strip. If there is enough Bovine TB antibodies in the sample, a visible T band will appear. The C band should always appear after a sample is applied, indicating a valid result. By this means, the device can accurately indicate the presence of Bovine Tuberculosis antibodies in the sample.

3. KIT COMPONENT

- 10×foil pouches, each containing a cassette, pipette and desiccant
- 1×assay buffer (4.0 mL)
- 20×pipette with reticle (pipette 1, 200μL, pipette 2, 20μL )
- 20×centrifugal tubes
- Product Manual

4.  TEST PROCEDURE

- Do centrifugation to cattle’s whole blood sample to collect serum or plasma for use.
- Use pipette 1 with reticle to transfer 200μL of assay buffer into a centrifugal tube.
- Use pipette 2 with reticle to transfer 20 μL (upper reticle) of serum or plasma into the centrifugal tube. Mix them well by the pipette attached in the pouch.
- Take out the cassette from the foil pouch and place it horizontally.
- Drip 3 drops (about 100 μL) of sample mixture into the sample hole.
- Interpret the result in 5 minutes. Result after 6 minutes is only considered as reference.

5. INTERPRETATION OF RESULTS
Positive: The presence of both C band and T band, no matter T band is clear or vague.
Negative: Only clear C band appears.
Invalid: No colored band appears in C zone, no matter whether T band appears.

6. STORAGE
The kit can be stored at room temperature (2-30°C). The test kit is stable through the expiration date (18 months) marked on the foil pouch. DO NOT FREEZE. Do not store the test kit in direct sunlight.

7. PRECAUTIONS
- For best results, please strictly adhere to these instructions.
- All reagents must be at room temperature before running the assay.
- Do not remove test cassette from its pouch until immediately before use.
- Do not reuse the test kit.
- Do not use the test beyond its expiration date marked on the foil pouch.
- The components in this kit have been quality control tested as standard batch unit. Do not mix components from different lot numbers.

8. LIMITATION

Quicking Bovine Tuberculosis Ab Rapid Test is for in vitro veterinary diagnosis use only. All results should be considered with other clinical information available from veterinarian. For an accurate result, It is suggested to apply other method such as ELISA for final determination in practice.