Liquid Based Cytology with Centrifugation and Precipitation Method/LBC with Automated Staining Slide System

                                           LTS-3000B LBC Slide Preparation and Staining System

Liquid-based cytology is a method of preparing samples for examination in cytopathology. The sample is collected, normally by a small brush, in the same way as for a conventional smear test, but rather than the smear being transferred directly to a microscope slide, the sample is deposited into a small bottle of preservative liquid. At the laboratory the liquid is treated to remove other elements such as mucus before a layer of cells is placed on a slide. The technique allows more accurate results. 

LTS-3000B is a breakthrough achievement of liquid based cytological techniques. The instrument combines density-gradient centrifugation, natural sedimentation and charge capture to complete the automation of specimen process, transfer, sedimentation, staining in one operation. 

Principles

1. Density-gradient Centrifugation.

The first centrifugation separates the diagnostic material from non-diagnosis debris like cell fragments and mucus; the second centrifugation enriches diagnostic material.

2.  Sedimentation and Separation.

The process & staining system employs cell diluent for natural sedimentation, since the gravity of lesion cells is higher than that of normal cell. As the settling rate of the former is faster, it will settle on slide in priory and combines with charges on slide. This feature is useful to collect the lesion cells at most extent and improve the positive detection ratio.

 3. Charge Capture.

The special polymer materials are used for slide process, which endows the slide surface with positive charges. The coat can capture cells with negative charges quickly and form the stable bonds; the captured cell quantity reaches to 5,000 - 50,000 per slide in case of white slide and cells dropping. 

4. Automated Staining.

The process & staining system performs full automated dripping staining with mechanical arm; each waste liquid from staining is discharged on time; after each staining is finished the mechanical arm flushes immediately in case of the cross contamination, the Pap and HE staining can be done quickly.

Operation Procedure

Ancillary Reagent

Slide Effect 

Initiated from 2000, Lituo Biotech is a national hi-tech enterprise specializing in R&D, production and marketing of high-quality in-vitro diagnostic reagents and clinical laboratory instruments. 

Lituo Biotech has strong R&D capability and leads the industry tendency. Lituo developed nearly 30 products including diagnostic reagents and instruments benefiting from its high calibre R&D teams, high investment of scientific research and efficient R&D management system.