Total Aflatoxins Immune Affinity Column

25 units/kit

Outline

Aflatoxin is a secondary metabolite produced by fungi such as Aspergillus flavus and parasitic aspergillus. It has strong carcinogenicity and is classified as class I carcinogen by WHO cancer research institute. At the same time, it is a highly toxic substance. It widely exists in various cereals, nuts, cottonseed and some products related to human blood and animal feed. Chinese. Aflatoxin M1, produced by the metabolism of aflatoxin B1 in feed, is still highly toxic and carcinogenic. It has been proved to be harmful to human and animal liver, kidney and other tissues and organs, and poses a great threat to human health. All countries in the world have strict limits on aflatoxins in food and feed.

Application 

It can specifically purify aflatoxins B1, B2, G1 and G2 in samples. Aflatoxin immunoaffinity column is widely used in the pretreatment of grain, food, feed, nuts and other samples.

Column capacity: > 200 ng

Recovery: > 80%

Principle

The basis of this experiment is the specific reaction between antigens and antibodies. The aflatoxin monoclonal antibody was coupled to the gel on the column. The aflatoxin in the sample was extracted, filtered and diluted. Then the sample extract was bonded to the affinity column with aflatoxin antibody. At this point, aflatoxin binds to the antibody in the affinity column. The impurities on the immunoaffinity column were then removed by distilled water. Aflatoxin was separated from the antibody by methanol elution affinity column. Finally, filtered methanol solution was injected into fluorometer or HPLC for determination.