Protein A Affinity Liquid Chromatography HPLC Column

Absolut A 
Protein A Affinity Columns For Monoclonal Antibody Analysis

Absolut A designed for fast analysis of monoclonal antibody (mAb) concentration (titer) in Affinity Chromatography. Recombinant protein A (rProtein A) has the advantages of bind the Fc region of immunoglobulins and alkali resistance. The spherical PS/DVB (Poly(styrene/divinylbenzene)) particles are highly cross-linked for enhanced mechanical stability, and bigger hole diameter. Compared with agarose base, hydrophilic PS/DVB has a higher dynamic binding capacity (DBC), longer lifetime and less shedding of rProtein A ligand. Hence, Absolut A is an excellent choice for mAbs analysis. 
 
Advantages 
• Direct use on HPLC instruments
• High dynamic binding capacity, quick mass transfer
• Minimum nonspecific absorption, accurate determination
• Fast analysis cycle time: 2-5 minutes
• Satisfactory linearity in wide concentration range: 0.02-10 mg/ml
• Long lifetime: approx. 3000 analysis cycles
• Alkali resistance: 0.1-0.5 M NaOH cleaning conditions

Main Characteristics 
Column Size:   2.0mm D×30mm L, 2.1mm D x100mm L, 4.6mm Dx50mm L, 4.6 mm D x100mm L 
Support Matrix:   Poly(styrene/divinylbenzene) (PS/DVB) 
Ligand:   Recombinant Protein A  
Particle Size:  30µm 
Dynamic Capacity (DBC):   Approx. 30 mg human IgG/ml media 
Shipping Solution:   0.02 M sodium phosphate, pH 7.0, 0.02% sodium azide 
pH range:   pH 2-10 
Maximum Operating Pressure:   1200 psi 
Cleaning Agents:   0.1-0.5M NaOH 
Cycle Time:   2-5 minutes 
Lifetime:   About 3000 analysis cycles 
Temperature Stability:   4-40 °C

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