Why does EDTA potassium inhibit ALP reaction

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Why does EDTA potassium inhibit ALP reaction

EDTA (ethylene diamine tetraacetic acid) is a commonly used chelating agent that can form complexes with many metal ions, thereby playing a chelating role. Among them, EDTA dipotassium is a form of EDTA, commonly used to suppress ALP reactions in blood collection and laboratory analysis to avoid interference with experimental results. So why can it be used to suppress ALP response? Below, we will give you a specific analysis.

1、 What is ALP

ALP, also known as alkaline phosphatase, exists in tissues such as the liver, bile duct, and bones of the human body and is very important. And it can catalyze the hydrolysis reaction of phosphate esters, converting one phosphate ion and one water molecule into two hydrogen ions and one phosphate ion. Therefore, ALP reaction is usually used to detect indicators such as bone growth and liver function.

2、 ALP reaction mechanism

The reaction mechanism is that it can combine with calcium ions under specific pH conditions to produce catalytic reactions. However, sometimes under certain experimental conditions, such as high or low environmental pH values during blood collection or experimental operations, or when some drugs or chemicals can affect the progress of ALP reactions, it is necessary to use EDTA potassium to achieve the experimental purpose.

3、 The Effect of EDTA Dipotassium on ALP

Due to the ability of EDTA dipotassium to form a stable complex with calcium ions (EDTA Ca2+), which prevents the binding process between calcium ions and ALP, the ALP reaction cannot proceed normally. In tests that require inhibition of ALP response, such as bone density measurement and liver function testing, EDTA dipotassium is an effective inhibitor, but it is usually used in conjunction with other reagents to maintain the stability and accuracy of experimental conditions.

The process of controlling ALP reaction with EDTA dipotassium is similar to the process of using it as an anticoagulant to prevent blood coagulation, basically controlling calcium ions to achieve the goal. However, attention should be paid to the quality issue of EDTA potassium dioxide. If the quality is poor and the performance is unstable, it will fail in any reaction process. As a professional manufacturer of anticoagulants, Desheng produces EDTA potassium dihydrate with a purity of up to 99%. The appearance of the crystal is uniform, and customers who have purchased it unanimously praise it. It will not interfere with the experiment and has become a hot selling product in the market. If you are interested, please click on the website to inquire and purchase!